Synonyms

Adrenocorticotrophin

Clinical Indications

Establishing the aetiology of proven Cushing's syndrome or differentiation between primary and secondary adrenal insufficiency (sample taken at 9.00 hrs). ACTH levels are not useful in monitoring steroid replacement therapy.

Availability

Analyzed by referral laboratory if specific criteria met.

Specific Criteria

Requested by Consultant Endocrinologists or if agreed with Consultant Biochemist for investigation of proven Cushing's or adrenal insufficiency.

Turnaround Time

2 weeks

Specimen

Plasma

Volume

2 ml

Container

Pink top (EDTA) tube

Collection

Samples should be taken between 09.00 and 10.00 hrs and transported to laboratory immediately.

Lab. Handling

Separate immediately into 2 aliquots and freeze at minus 20’C. (ACTH & send; NOACT & save)

Causes for Rejection

Not meeting specific criteria for analysis. Delay in sample reaching laboratory

Reference Range

 09.00 hrs sample: Less than 50 ng/L