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Clinical Biochemistry Sample Requirements

Blood Samples
Most routine Clinical Biochemistry blood tests can be undertaken on 6 ml. sample collected into a Vacutainer SST tube (yellow top). Some common exceptions are:

N.B. Haemolysed and lipaemic blood samples may interfere with analysis in a significant and unpredictable manner requiring caution with interpretation of results. Information on the effects of haemolysis on routine Clinical Biochemistry tests is available.

Glucose Samples
Glucose analysis may be undertaken on unpreserved samples, but the result may be lowered if the sample is not analysed within 4 hours, or if glucose is being metabolised rapidly (e.g. in septicaemia or some forms of leukaemia). Under these circumstances, a 2ml samples in a fluoride oxalate tube (grey top) is required

Alcohol Samples
Requests for alcohol are not routinely available without prior discussion with the laboratory. A 2ml samples in a fluoride oxalate tube (grey top) is required.

Paediatric Samples
Paediatric samples for most Clinical Biochemistry tests may be collected into a 2 ml heparinised micro tube (orange cap) available from Pathology: 2 ml fluoride oxalate micro tubes are also available for glucose.

Blood Gases
All blood gas samples are analysed 'Point of Care' and require an arterial sample taken into an heparinised syringe.

Urine Samples
Plain 25ml containers (white top) are available from supplies distribution for random, MSU and EMU samples. 24 hour urine containers with any necessary preservative, are available from Pathology. Information on urine collection and preservatives required is available.

Glycated Haemoglobin (Hba1c)
Hba1c requests require a separate 2 ml blood sample taken into a Vacutainer EDTA tube (purple top).

Faecal Samples
Plain 25ml faecal containers (blue top) are available from supplies distribution.

Special Collection Conditions Required

Test Name Sample Type Container Comments

Aldosterone

Plasma

Plastic Heparin  

Contact laboratory. Samples must be transported to lab. immediately.

Ammonia

Plasma

Paediatric Orange Top

Contact laboratory. Samples must be transported to lab. immediately..

Calcitonin

Plasma

Plastic Heparin  

Contact laboratory. Samples must be transported to lab. immediately.

Gut hormones

Plasma

Heparin with Trasylol

Contact laboratory. Samples must be transported to lab. immediately.

Homocysteine

Plasma

EDTA (Purple Top)

Samples must be transported to lab. within 30 minutes.

Porphyrins

Urine / Plasma / Faeces

Plain (white top) universal
Purple top (EDTA) tube
or (blue top) universal

Keep sample in dark until delivery to laboratory.

Troponin T

Serum

Yellow Top (SST). Do not use heparin. 

Must be taken at least 12 hours after event

Xanthochromia

CSF

White Capped Universal

Must be taken at least 12 hours after event. A minimum of 1ml of CSF is required.

Last edited 07/08/08